The purpose:
Demonstrate how the ELISA assay is used to diagnose exposure to a disease by testing for the presence of antibodies to the disease in a sample of simulated serum.
Determine the hemagglutinating antibody titer of two different sera.
Demonstrate Complement Mediated Lysis and the sequence of reactions necessary to cause lysis.
Bench setup
Student bench setup
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- Rack 1: 5 x empty 15ml tubes for Complement Lysis, 2 x 10ml tubes with saline, 1 x 10ml tube with 1%SRBC
- Rack 2: Complement Lysis reagents
- Rack 3: Elisa reagents
- Biorad 12 well strip
- Stack of brown paper
- 96 U-bottom well plate and towels
seal for hemagglutination
- Wash buffer with plastic
1 ml transfer pipette
- Liquid Waste container
- Bio-hazard waste
- Timer
- 5 ml serological pipette and Pi-pump
- 2x Markers (fine tip)
- Pasteur pipettes and bulbs
- Adjustable 1000 µl and 200 µl pipettes and tips
- Centrifuge
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Front bench setup
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- Water bath at 37 °C + plastic racks
- Tray to place hemagglutination plates going to Incubator
- Bio-hazard waste
- 200 µl pipette tips
- Serum 1
- Serum 2
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