Intro:

In clinical practice, hematological information accumulated from a series of blood tests conducted on a small volume of blood - even a single drop - can be of great diagnostic and prognostic value. Of the various kinds of simple blood tests available, three groups have been selected for this laboratory session:
   - Erythrocyte Fragility
   - Erythrocyte Sedimentation Rate (ESR)
   - Hemostatic Tests
   - Blood Cell Indices
To derive the most benefit from this exercise, students should review appropriate chapters in the textbook dealing with the various constituents of blood, their functional characteristics, and the regulatory factors that determine their numbers or concentrations.

Student bench setup
Centrifuge Spectrophotometer Kim wipes Centrifuge tubes (6) with saline solutions cuvettes (6) RBC (Gower’s) dilution fluid and blood sample (X, Y or Z) Heparinized capillary tube (1) “Seal-ease” (1) Biohazard bag Hemocytometer (1) and cover Slip (2) Artificial blood + 3 antiserums for blood typing + tray for mixing Timer Pasteur pipettes (6) with 2 bulbs Markers (2) P20 and P200 pipettes + tips Lab manuals (2) Student microscope with counter and immersion oil
Front bench setup
Sedirack - prepare in advance and keep. Cover with film and keep at 4 °C Hemoglobinometer + cartridges Hematocrit Centrifuge (1) Difquik stained slides - in boxes (X,Y, Z) 3 x WBC blood samples for X (1). Y (1) and Z (1) -100 µl /tube WBC dilution fluid 100 µl / patient (bench)