The McGill Physiology Virtual Lab

Exercise Physiology Laboratory

Exercise Protocol
 

In the laboratory, several sessions of three minutes of stepping predetermined cadences based on the age and gender of the subject will be performed, as implemented in the modified Canadian Aerobic Fitness Test (mCAFT).
T
he heart rate at the end of each stepping stage is recorded (the subject wears a Polar heart monitor).
The subject continues to the next stage if the attained heart rate is under 85% of predicted (predicted maximum heart rate = 220—age).
At the start of the second minute of each stage, the subject breathes through a Spirometer for 20 seconds, such that tidal volume and breathing frequency can be recorded and minute ventilation can be calculated
.
VO2Max is estimated,  and oxygen cost and caloric expenditure for that exercise is calculated.

The modified Canadian Aerobic Fitness Test (mCAFT)
 

 

The Canadian Society for Exercise Physiology has published "The Canadian Physical Activity, Fitness & Lifestyle Approach", which details fitness assessment protocols. One such protocol, the mCAFT, is designed to give information about the aerobic fitness of a person, while using minimal equipment. The subject works by lifting its own body weight up and down double steps (40.6 cm in height total) while listening to set cadences from a compact disc. The end-stage of the age and gender specific stepping rate requires 85% of the age-predicted maximum heart rate. The heart rate increases approximately in a linear fashion from 50% to 100% of maximal oxygen intake. The heart rate does not decrease significantly during the first fifteen seconds of recovery. Thus, one can predict an aerobic fitness using the heart rate right after exercise of a known sub-maximal rate of working.

Starting stepping stage by gender

Age Males Females
15-19 4 3
20-29 4 3

 

Oxygen cost in ml/kg/min

Stage Stepping cadence (Females) Stepping cadence (Males) oxygen cost (Females) Oxygen cost (Males)
3 102 102 22 22
4 114 114 24.5 24.5
5 120 132 26.3 29.5
6 132 144 29.5 33.6
7 118 132 36.2 40.1
Stepping routine  
The subject is standing facing the steps

Step- step up


Place right foot on first step

Place left foot on second step

Place right foot on second step so feet are together
 

Step- step down


Start down with your left foot to the first step

Place right foot on ground level

Place your left foot on ground so feet are together
 
Each stepping session lasts for three minutes. At the beginning of the second minute of each stage, the individual will breathe for 20 seconds to record tidal volume and breathing frequency and calculate minute ventilation.
Pneumotachometry
  • Refer to the pneumotachometry section of the respiratory lab (other experiments) to view the setup used to record the tidal volume.


  • The subject must wear a nose clip while breathing into the flowhead.
  • A fresh mouthpiece is used. The mouthpiece must be placed inside the mouth so that only the tube part shows.
Heart rate monitoring
A Polar (www.polar.fi) heart rate monitor (belt and watch) is used to measure heart rate continuously. A transmitter belt is fastened around the chest while the watch is held by a nearby observer. If at any time during the experiment the heart rate exceeds the predetermined ceiling (85% of age-predicted max heart rate) the experiment should be stopped immediately.
Blood pressure measurement
Systolic and diastolic blood pressures are recorded using an automated sphygmomanometer before starting the first workload and at 2 minutes and at 5 minutes post recovery.
Calculations  
The predicted VO2Max is derived from this equation*:

17.2 + (1.29 x oxygen cost at the final stage) - (0.09 x weight in kg) - (0.18 x age in years)

* Weller et al. Prediction of maximal oxygen uptake from a modified Canadian aerobic fitness test. Can. J. Appl. Physiol. 18(2) 175-188, 1993

*Weller et al. A study to validate the Canadian aerobic fitness test. Can. J. Appl. Physiol. 20(2) 211-221, 1995