The McGill Physiology Virtual Lab

Cardiovascular Laboratory

ECG> Experiments
  The ECG trace depends on the lead configuration, which sets the orientation of the lead axis relative to the heart. The standard limb leads have the disadvantages that the three electrodes are all in the same plane (frontal plane) of the body, so that one is only recording a projection of the three-dimensional spread of depolarization and repolarization in that plane.
 
1) Inspecting the ECG
  • While displaying Lead II, the P-wave, the QRS-complex, and the T-wave are identified.  In the chart program, experiment with different settings for the time scale and the voltage scale so that, e.g. the waveform is not too big or small in amplitude.

 

 
2) Identifying Waves and Intervals
  • The time scale is set to an appropriate value for displaying the overall shape of the P-QRS-T waveform.  The various complexes and intervals are examined.  The RR, PR, QRS and QT intervals are measured, and compared to the normal ranges given in the table to the right (in seconds).
Interval Min Max
RR 0.6 1.2
PR 0.12 0.20
QRS   0.10
QT   0.42

 

3) Effect of Lead Placement

  • The white (Neg) electrode on the right arm is moved from its position on the wrist to a new position somewhere above the elbow.
For convenience, the connections of the ECG electrodes are usually made at the ends of the limbs: at the wrists and ankles.  However, since the limbs act as conductors, they can be viewed as an extension of the patient cable lead, and so it makes no difference where the electrodes are placed along the limb length.
  • After returning the white electrode to its original position, the subject extends the right arm outwards and holds it horizontally in mid-air away from the body.
  The above ECG trace appears very noisy, because the recording is also picking up the EMG activity from the muscles used in extending the arm outwards.

4) Effect of Respiration

  • The subject takes a deep slow breath, and then exhales slowly (inhaling for 5 seconds, and exhaling for five seconds).
In sinus arrhythmia, the heart rate varies with the phase of respiration.  The heart rate typically increases during inspiration and decreases during expiration.  Therefore, as observed, the R-R interval is longer during expiration.  These changes are mediated through vagal reflexes.  Sinus arrhythmia is more common in young healthy athletes.

5) The Timing of the Heart Sounds

  • One member of the group listens with the stethoscope to the subject's heartbeat to determine where the two well-separated heart sounds fall on the ECG trace.
 
  The first heart sound S1 is due to the closure of the mitral and tricuspid valves at the start of ventricular systole.  The second heart sound S2 is due to the closure of the aortic and pulmonary valves. Click here for more on the heart valves

6) Changes in Morphology with Leads

  • The cables are connected so as to record from lead I.  The group should describe the changes seen with respect to lead II, and attempt to explain them.
  • The cables are connected so as to record from lead III.  The group should describe the changes seen with respect to lead II, and attempt to explain them.
  • The three following leads were recorded in one particular individual. There is no guarantee that the ECG recorded by your group will be similar to those three traces, since there is large inter-individual variability in the ECG.

Recall that the R wave is due to the activation (depolarization) of the major portion of the ventricles.  From the sample data above, it is evident that the lead whose axis is most parallel to the direction of the subject's ventricular depolarization is lead II. (The R wave is largest in lead II.)   The R wave is very small in lead I.  We can therefore conclude that for this subject the direction of ventricular depolarization is more close to being perpendicular to lead I.
To continue with the next section: ECG Disorders, click here