Instrument Standardization and Quality Control
Director of the McGill University Life Sciences Complex Imaging Facility we
are developing protocols and standards for testing the quality of light
microscopes. We have developed protocols for measuring laser stability,
microscope alignment, resolution, and objective lens quality. We are leading
world wide studies on instrument quality and publishing detailed protocols
enabling microscopists world-wide to validate and maintain their equipment.
These tests are applied routinely to all of the microscopes within the
facility ensuring researchers are working on top performing equipment.
The facility has
13 state-of-the art light microscopy platforms used by ~150 users from
60 laboratories across Montreal to conduct their microscopy based
research. The facility has expertise in many areas including live cell
imaging, total internal reflection fluorescence (TIRF) microscopy,
fluorescence lifetime imaging microscopy (FLIM), multi-photon
microscopy, laser micro-dissection, fluorescence correlation
spectroscopy (FCS), image processing and analysis, spectral imaging and
high content screening. We have also developed and run more than 30
workshops and courses, in collaboration with corporations in the field
of light microscopy, including the inaugural Montreal Light Microscopy
Course (MLMC) in 2010. We are busy planning MLMC 2012 for July 9-20,
J. A., Lin, Rappaz, B., Webb, D. J., Brown, C. M.
“Intra-molecular Fluorescence Resonance Energy Transfer (FRET) Microscopy”
Nat. Prot., In Press.
Broussard, J. A., Lin, W. H., Majumdar, D., Anderson, B., Eason, B.,
Brown, C. M. & Webb, D. J. “The endosomal adaptor protein APPL1
impairs the turnover of leading edge adhesions to regulate cell migration.”
Mol Biol Cell 23, 1486-1499 (2012).
Lacoste, J., Vining, C., Zuo, D.,
Spurmanis, A., Brown, C.M. (2012) “Optimal Conditions for
Live Cell Microscopy and Raster Image Correlation Spectroscopy (RICS)”
Chapter in Annual Reviews in Fluorescence 2010, Editor Chris D. Geddes.
Lacoste, J., Young, K. and
Brown, C. M. (In Press) “Live-cell Migration and Adhesion
Turnover Assays”, Cell Imaging Techniques, Volume 2, Editor Dr. Douglas
Taatjes. Humana Press.
Webb, D. J. and Brown, C.
M. (In Press) “Epi-fluorescence Microscopy”, Cell Imaging
Techniques, Volume 2, Editor Dr. Douglas Taatjes. Humana Press.
Aswani, K., Jinadasa, T.,
Brown, C. M., “Fluorescence microscopy Light Sources” Microscopy
Today, 20(4), (2012).
R.W., Jinadasa, T., Brown, C.M., (2011) "Measuring and
Interpreting Point SpreadFunctions to Determine Confocal Microscope
Resolution and Ensure Quality Control."
6, 1929-1941 (2011)
(2011) " Phenylpyrrolocytosine-modified siRNA: Unobtrusive base modification
for monitoring activity and cellular trafficking", ACS Chemical
Epub June 21, 2011.
Frigault, M. M., Lacoste, J., Swift, J.L., Brown, C. M.
(2009) "Live-cell Microscopy: Tips and Tools." J. Cell Sci.
M.E., C.M. Brown, N. Lamarche-Vane, and S. Richard. (2009)
"An adaptor role for cytoplasmic Sam68 in modulating Src activity during
cell polarization." Mol Cell Biol. 7:1933-43.
Brown, C. M., Dalal, R. B., Hebert, B., Digman, M. A.,
Horwitz, A. F., Gratton, E. (2008) “Raster Image Correlation Spectroscopy
(RICS) Measuring Fast Protein Dynamics and Concentrations with a Commercial
Laser Scanning Confocal Microscope” J. Microscopy, 229,
Digman, M.A.*, Brown, C. M.*, Horwitz, A. F., Mantulin, W.
W., and Gratton, E. (2008) “Paxillin Dynamics Measured During Adhesion
Assembly and Disassembly by Correlation Spectroscopy.” Biophys. J.,
94(7), 2819-2831. * Equal Contribution