Department of Physiology, McGill University, Montreal, Quebec, Canada
 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

PUBLICATIONS

Alexander, R.T., Malevanets, A., Durkan, A.M., Kocinsky, H.S., Aronson, P.S., Orlowski, J. and Grinstein S. (2007) Membrane curvature alters the activation kinetics of the epithelial Na+/H+ exchanger NHE3.  J. Biol. Chem. 282(10):7376-84 

Alexander, R.T., Furuya, W., Szaszi, K., Orlowski, J., and Grinstein, S. (2005) Rho GTPases dictate the mobility of the Na+/H+ exchanger NHE3 in epithelia. Role in apical retention and targeting.  Proc. Natl. Acad. Sci. USA 102(34): 12253-12258. 

Kocinsky, H.S., Girardi, A.C.C., Biemesderfer, D., Nguyen, T., Mentone, S.A., Orlowski, J., and Aronson, P.S. (2005) Use of phosphospecific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.  Am. J. Physiol. Renal Physiol. 289: F249-F258.

Lin, P. J. C., Williams, W. P., Luu, Y., Molday, R. S., Orlowski, J. and Numata, M. (2005) Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7.  J. Cell Sci. 118: 1885-1897. 

Szabó, E.Z., Numata, M., Lukashova, V., Iannuzzi, P. and Orlowski, J. (2005) b-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform.  Proc. Natl. Acad. Sci. USA 102(8): 2790-2795.

Orlowski, J. and Grinstein, S. (2003) Diversity of the mammalian sodium/proton exchanger SLC9 gene family. Pflügers Archives - Eur. J. Physiol. (in press)

Szászi, K., Paulsen, A., Szabó, E.Z., Numata, M., Grinstein, S., and Orlowski, J. (2002) Clathrin-mediated endocytosis and recycling of the neuron-specific Na+/H+ exchanger NHE5 isoform: regulation by phosphatidylinositol 3’-kinase and the actin cytoskeleton. J. Biol. Chem. 277 (45): 42623-42632.

Hayashi, H., Szászi, K., Coady-Osberg, N., Orlowski, J., Kinsella, J. L., and Grinstein, S. (2002) A slow pH-dependent conformational transition underlies a novel mode of activation of the epithelial Na+/H+ exchanger NHE3 isoform. J. Biol. Chem. 277 (13): 11090-11096.

Khadilkar, A., Iannuzzi, P., and Orlowski, J. (2001) Identification of sites in the second exomembrane loop and ninth transmembrane helix of the mammalian Na+/H+ exchanger important for drug recognition and cation translocation. J. Biol. Chem. 276 (47): 43792-43800.

Szászi, K., Kurashima, K., Kaibuchi, K., Grinstein, S., and Orlowski, J. (2001) Role of the cytoskeleton in mediating cAMP-dependent protein kinase inhibition of the epithelial Na+/H+ exchanger NHE3. J. Biol. Chem. 276 (44): 40761-40768.

Numata, M. and Orlowski, J. (2001) Molecular cloning and characterization of a novel (Na+, K+)/H+ exchanger localized to the trans-Golgi network. J. Biol. Chem. 276(20): 17387-17394.

Aharonovitz, O., Kapus, A., Szászi, K., Coady-Osberg, N., Jancelewicz, T., Orlowski, J. and Grinstein, S. (2001) Modulation of Na+/H+ exchange activity by Cl-. Am. J. Physiol. 281: C133-C141.

Denker, S. P., Huang, D. C., Orlowski, J., Furthmayr, H., and Barber, D. L. (2000) Direct binding of the Na-H Exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H+ translocation. Molecular Cell 6: 1425-1436.

Szászi, K., Kurashima, K., Kapus, A., Paulsen, A., Kaibuchi, K., Grinstein, S. and Orlowski, J. (2000) RhoA and Rho kinase regulate the epithelial Na+/H+ exchanger NHE3. Role of myosin light chain phosphorylation. J. Biol. Chem. 275: 28599-28606.

Aharonovitz, O., Zaun, H. C., Balla, T., Orlowski, J. and Grinstein, J. (2000) Intracellular pH regulation by Na+/H+ exchange requires phosphatidylinositol 4,5-bisphosphate. J. Cell Biol. 150: 213-224.

Szabó, E. Z., Numata, M., Shull, G. E., Orlowski, J. (2000) Kinetic and pharmacological properties of human brain Na+/H+ exchanger isoform 5 stably expressed in Chinese hamster ovary cells. J. Biol. Chem. 275: 6302-6307.

Chow, C.-W., Khurana, S., Woodside, M., Grinstein, S. and Orlowski, J. (1999) The epithelial Na+/H+ exchanger, NHE3, is internalized through a clathrin-mediated pathway. J. Biol. Chem. 274: 37551-37558.

Kurashima, K., D’Souza, S., Szaszi, K., Ramjeesingh, R., Orlowski, J., and Grinstein, S. (1999) The apical Na+/H+ exchanger isoform NHE3 is regulated by the actin cytoskeleton. J. Biol. Chem. 274: 29843-29849.

Chow, C. W., Woodside, M., Demaurex, N., Yu, F. H., Plant, P., Rotin, D., Grinstein S., and Orlowski, J. (1999) Proline-rich motifs of the Na+/H+ exchanger NHE2 isoform: Binding of SH3 domains and role in apical targeting in epithelia. J. Biol. Chem. 274: 10481-10488.

Baird, N.R., Orlowski, J., Szabó, E. Z., Zaun, H.-C., Schultheis, P.J., Menon, A.G., and Shull, G.E. (1999) Molecular cloning, genomic organization, and functional expression of Na+/H+ exchanger Isoform 5 (NHE5) from human brain. J. Biol. Chem. 274: 4377-4382.

Petrecca, K., Atanasiu, R., Grinstein, S., Orlowski, J., Shrier, A. (1999) Subcellular localization of the Na+/H+ exchanger NHE1 isoform in rat myocardium. Am. J. Physiol. 276: H718-H724.

Kurashima, K., Szabó, E. Z., Lukacs, G., Orlowski, J., and Grinstein, S. (1998) Endosomal recycling of the Na+/H+ exchanger NHE3 isoform is regulated by the phosphatidylinositol 3-kinase pathway. J. Biol. Chem. 273: 20828-20836.

D’Souza, S., Cabado, A. G., Yu, F. H., Teter, K., Lukacs, G., Skorecki, K., Moore, H. P., Orlowski, J., and Grinstein, S. (1998) The epithelial sodium-hydrogen antiporter Na+/H+ exchanger 3 accumulates and is functional in recycling endosomes. J. Biol. Chem. 273: 2035-2043.

Kurashima, K., Yu, F. H., Cabado, A. G., Szabó, E. Z., Grinstein, S., Orlowski, J. (1997) Identification of sites required for down-regulation of Na+/H+ exchanger NHE3 activity by cAMP-dependent protein kinase: Phosphorylation-dependent and -independent mechanisms. J. Biol. Chem. 272: 28672-28679.

Orlowski, J. and Grinstein, S. (1997) Na+/H+ exchangers in mammalian cells. J. Biol. Chem. 272: 22373-22376.

Roberston, M. A., Woodside, M., Foskett, J. K., Orlowski, J., and Grinstein, S. (1997) Muscarinic agonists induce phosphorylation-independent activation of the NHE-1 Isoform of the Na+/H+ antiporter in salivary acinar cells. J. Biol. Chem. 272 (1): 287-294.

Demaurex, N., Romanek, R.R., Orlowski, J., and Grinstein, S. (1997) ATP dependence of Na+/H+ exchange: nucleotide specificity and assessment of the role of phospholipids. J. Gen. Physiol. 109: 117-128.

Lin, X., Voyno-Yasenetsakaya, T., Hooley R., Lin, C.-Y., Orlowski, J., and Barber, D. L. (1996) G12 differentially regulates Na+-H+ exchanger isoforms. J. Biol. Chem. 271: 22604-22610.

Orlowski, J. and Kandasamy, R.A. (1996) Delineation of transmembrane domains of the Na+/H+ exchanger that confer sensitivity to pharmacological antagonists. J. Biol. Chem. 271: 19922-19927.

Azarani, A., Goltzman, D., and Orlowski, J. (1996) Structurally diverse N-terminal peptides of parathyroid hormone (PTH) and PTH-related peptide (PTHRP) inhibit the Na+/H+ exchanger NHE3 isoform by binding to the PTH/PTHRP receptor type I and activating distinct signaling pathways. J. Biol. Chem. 271: 14931-14936.

Kandasamy, R.A. and Orlowski J. (1996) Genomic organization and glucocorticoid transcriptional activation of the rat Na+/H+ exchanger Nhe3 gene. J. Biol. Chem. 271: 10551-10559.

Cabado, A.G., Yu, F. H., Kapus, A., Gergely, L., Grinstein, S., and Orlowski, J. (1996) Distinct structural domains confer cAMP sensitivity and ATP dependence to the Na+/H+ exchanger NHE3 isoform. J. Biol. Chem. 271: 3590-3599.

Kandasamy, R.A., Yu, F. H., Harris, R., Boucher, A., Hanrahan, J.W., and Orlowski, J. (1995) Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways. J. Biol. Chem. 270: 29209-29216.

Azarani, A., Orlowski, J., and Goltzman, D. (1995) Parathyroid hormone and parathyroid hormone-related peptide activate the Na+/H+ exchanger NHE-1 isoform in osteoblastic cells (UMR-106) via a cAMP-dependent pathway. J. Biol. Chem. 270: 23166-23172.

Azarani, A., Goltzman, D., and Orlowski, J. (1995) Parathyroid hormone and parathyroid hormone-related peptide inhibit the apical Na+/H+ exchanger NHE-3 isoform in renal cells (OK) via a dual signaling cascade involving protein kinase A and C. J. Biol. Chem. 270: 20004-20010.

Orlowski, J. (1993) Heterologous expression and functional properties of the amiloride high affinity (NHE-1) and low affinity (NHE-3) isoforms of the rat Na/H exchanger. J. Biol. Chem. 268: 16369-16377.

Kapus, A., Grinstein, S., Wasan, S., Kandasamy, R., and Orlowski, J. (1994) Functional characterization of three isoforms of the Na+/H+ exchanger stably expressed in Chinese hamster ovary cells: ATP dependence, osmotic sensitivity and role in cell proliferation. J. Biol. Chem. 269: 23544-23552.

Yu, F., Shull, G.E., and Orlowski, J. (1993) Functional properties of the rat Na/H exchanger NHE-2 isoform expressed in Na/H exchanger-deficient Chinese hamster ovary cells. J. Biol. Chem. 268: 25536-25541.

Wang, Z., Orlowski, J., Shull, G.E. (1993) Primary Structure and functional expression of a novel gastrointestinal isoform of the rat Na/H exchanger. J. Biol. Chem. 268: 11925-11928.

Orlowski, J., Kandasamy, R.A., and Shull, G.E. (1992). Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins. J. Biol. Chem. 267: 9331-9339.

 

This page was last edited on 01 June, 2007